ABSTRACT
Background: During the last decade Revista Médica de Chile increased its visibility, measured on citations and impact factor. Aim: To perform a scientometric analysis to assess the performance of Revista Médica de Chile. Material and methods: Thomson's-ISI Web of Science and Journal Citation Reports QCR) were consulted for performance indicators of Revista Médica de Chile and Latin Amerícan journals whose subject is General and Internal Medicine. We also report the h-index of the journal, which infers quality ¡inked to the quantity of the output. Results: According to the h-index, Revista Médica de Chile ranks 4 among the 36 journals indexed and published by Argentina, Brazil, Chile and México. The top ten articles published by Revista Médica de Chile and the institutions with the higher contríbution to the journal, were identified using citations. In the Latin Amerícan región, Brazil relevantly increased its scientific output. However, Argentina, Chile and México maintain a plateau during the last decade. Conclusions: Revista Médica de Chile increased notoriously its performance. Its contríbution to the Chilean scientific community dedicated to Medicine appears to be of central value.
Subject(s)
Bibliometrics , Biomedical Research/statistics & numerical data , Periodicals as Topic/statistics & numerical data , Biomedical Research/standards , Chile , Databases, Bibliographic , Internal Medicine , Journal Impact Factor , Latin America , Periodicals as Topic/standards , PublishingABSTRACT
The seasonal adaptation of the teleost Cyprinus carpio to the cyclical changes of its habitat demands physiological compensatory responses. The process involves profound nucleolar adjustments and remarkable changes in rRNA synthesis, which affects ribosomal biosynthesis. In this context, we have demonstrated that the synthesis of several proteins involved in ribosomal biogenesis as protein kinase CK2, ribosomal protein L41 and nucleolin, as well as U3 snoRNP, are differentially regulated in summer-acclimatized carp compared to the cold-season adapted fish. To understand the mechanisms involved in the seasonal regulation of rRNA gene transcription, we have been studying the carp rDNA cistron structure. Because the cis-elements that regulate the expression of the tandem organized ribosomal genes are located in the non-transcribed intergenic spacer (IGS), we analyzed the primary structure of the carp rDNA gene IGS. The gene organization is similar to that described from other vertebrate species, including numerous repetitive sequences, the transcription start site, and some potential cis-elements such as ribosomal enhancers, proximal terminator and transcriptional terminators. Ribosomal DNA is a remarkable case of gene duplication and has been used as a model to test the concerted evolution theory. We performed sequence comparison analyses of 18S rRNA coding sequences from carp with different species, data with which an unrooted phylogram was constructed
Subject(s)
Animals , Male , Carps , Genes , RNA, Ribosomal , Gene Expression Regulation , Gene Library , Molecular Sequence Data , Phylogeny , Repetitive Sequences, Nucleic Acid , RNA, Ribosomal , SeasonsABSTRACT
During the last decade the articles published by Chilean Research Centers grew 1,73 which compares to the 2.34 fold increase of mainstream research articles registered as a whole in Latin America. However, the relative impact of the Chilean publications surpassed that of Latin America. In Biological Sciences, traditionally the strongest research area within Chile, Latin America also shows a steeper slope of growth. Qualitatively, biological disciplines in Chile are comparable to those published in Latin America although in Chile there are specialties as Physiology that surpass the average world's impact. The scientometric data is consistent with the fall in individual grants that the Chilean Research Fund (FONDECYT) has been allocating during the last decade.
Subject(s)
Bibliometrics , Biological Science Disciplines , Publishing , Research , Chile , PeriodicalABSTRACT
We isolated and cloned a carp somatolactin SL DNA fragment, of which 78 per cent of the nucleotides were identical to the corresponding salmon SL sequence. The results obtained upon Northern blot hybridization of carp pituitary RNA allowed the identification of two transcripts as described for other fish. When the content of SL transcripts in pituitary sections from summer- and winter-acclimatized carp was quantified by in situ hybridization assays, we found no significant differences between the two seasons. In salmonids, plasma SL reaches higher levels in summer than in winter in synchrony with the water temperature cycle; in the eurythermal carp, however, the complex adaptive responses imposed by seasonal environmental changes do not seem to include the regulation of the somatolactin detected with the probe used at the transcriptional level in pituitary glands.
Subject(s)
Animals , Acclimatization , In Situ Hybridization/methods , Pituitary Gland , Pituitary Hormones/genetics , Transcription, Genetic , Base Sequence , Blotting, Northern , Carps , DNA Fragmentation , Pituitary Hormones/isolation & purification , SeasonsABSTRACT
Scientific productivity in Chile was studied examining a data bank constructed with the publications indexed by the Institue for Scientific Information during 1987 and 1988. the bank excludes meeting abstracts and contains the full title of the publisched paper, the list of authors, addresses, field, and the nature of collaboration two or more institutions. The articles were classified in different fields and tabulated according to the institution from which they originated. Although remains to be the more productive subjective (26.5%) followed by medical sciences (23.9%) and chemistry (12.3%), articles in mathematics and physics continued theri increase as in previous years. Using the scientometric indicators published by Braun et al (see refs. 14-17), the impact of the research originated in Chile in biology, physics and mathematics was compared to that attained in Argentina, Brazil, Mexico and Venezuela. The role of the chilean National Research Fund and the output of the financed resarch projects were also analyzed. The succesful results obtained during the first years in which the National Research Fund has been involved in the support of the scientific activity in Chile, confirms the need to strenghten its budget, according to the foals stated in the National Plan for Science and Technology for Development
Subject(s)
Publishing/statistics & numerical data , Chile , Publishing/classification , Research , Research Support as TopicABSTRACT
Se sintetizó un análogo fotoactivable de biotina, el que se utilizó para marcar sondas de ácidos nucleicos. La marca se reveló con dos sistemas de detección avidina-peroxidasa y estreptavidina-fosfatasa alcalina, siendo ésta última la que demostró una mayor sensibilidad. Los plasmidos pSS1.8 y pSP64/U1 fueron fotobiotinilados y utilizados en ensayos de hibridación en gota con DNA extraido de leucocitos humanos. Despues de la incubacion con estreptavidina y fosfatasa alcalina biotinilada, la actividad de la enzima se reveló con un sustrato soluble. Los resultados obtenidos demuestran diferencias cuantitativas consistentes con el número de copias para globina y U1snRNA humano. El plasmido pSS1.8 fotobiotinilado se utilizó para identificar fragmentos de restricción de DNA genómico alterados en un paciente afectado de anemia de células falciformes. El gen de la globina mutado se detectó por digestión del DNA del paciente con la endonucleasa de restricción Dde I, seguido de una hibridación "Southern" con la sonda marcada